|
KMID : 0355420100340040621
|
|
Journal of Korean Academy of Oral Health
2010 Volume.34 No. 4 p.621 ~ p.627
|
|
|
Influence on enamel surface by the various tooth storage methods
|
|
Jeong Seung-Hwa
Kwon Ho-Keun
Kim Baek-Il
|
|
|
Abstract
|
|
|
Objectives.The aim of this study was to assess the influence of storage methods on the enamel specimen.
Methods.Each enamel specimen (3 mm in diameter) from bovine anterior teeth was stored in 5 mL of 6 kinds of media which were saline, distilled water, thymol solution (0.1%), Hank’s balanced salts solution (HBSS), ethanol (70%) and frozen at ?20oC for 25 and 125 days. In order to evaluate effects of each storage method, surface micro hardness (VHN) of enamel surface was measured before and after storage of specimens. Also, confocal laser scanning microscope (CLSM) and polarized light microscope (PLM) were used to observe the sagittal section of the enamel specimen.
Results.After storage for 25 days in the saline, distilled water, 0.1% thymol solution and HBSS media, the micro hardness of the enamel surface have decreased significantly (p＜0.01). In contrast, storing teeth in 70% ethanol and freezing at ?20oC did not have significant difference in the micro hardness before and after storage. After storage for 125 days, only freezing at ?20oC showed no storage effect to the enamel surface.
Conclusions.It was concluded that 70% ethanol and freezing at ?20oC with no surface contact of water could be proper methods for storage of teeth in micro hardness study. So, we recommend that extracted teeth and tooth specimens should be shortly stored in the 70% ethanol for disinfection process, and then be stored with freezing temperature of ?20oC with no surface contact of water.
|
|
|
KEYWORD
|
|
|
bovine enamel surface, demineralized depth, microhardness, tooth storage method, -20℃, 70% ethanol
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|
|